Donor substrate specificity of bovine kidney gamma-glutamyltransferase
نویسندگان
چکیده
منابع مشابه
Donor substrate specificity of bovine kidney gamma-glutamyltransferase.
The enzyme γ-glutamyltransferase (GGT) catalyzes the hydrolysis of the γ-glutamyl isopeptide bond of glutathione conjugates (donor substrates), releasing glutamic acid, or the transfer of the donor's γ-glutamyl group to an acceptor substrate, such as a dipeptide. The specificity of GGT for xenobiotic donor substrates has not been fully characterized. The transpeptidation activity of bovine kidn...
متن کاملGamma-glutamyltransferase: Substrate inhibition, kinetic mechanism, and assay conditions.
Gamma-glutamyltransferase activity in serum is shown to be competitively inhibited by the two substrates gamma-glutamyl-4-nitroanilide and glycylglycine. Awareness of this is of importance when one is choosing final reaction conditions for the assay of the enzyme. Gamma-glutamyltransferase probably acts by a "ping-pong bi-bi" kinetic mechanism, which fits with the double competitive substrate i...
متن کاملThe isolation and substrate specificity of a bovine kidney enzyme cleaving L-prolylvalylglycine [proceedings].
three regions of peptidase activity at RF 0.03, 0.06 and 0.25 did not co-migrate with stained protein bands on the gel. The slowest-migrating peptidase hydrolysed diand tri-peptides and was frequently observed as a double peak of activity near the origin of the gel. The faster moving diand tri-peptidase was found at a narrow unstained region of the gel between bands 2 and 3. The dipeptidase enz...
متن کاملMarkedly elevated gamma-glutamyltransferase.
Gamma-glutamyltransferase (GGT) is a highly sensitive but poorly specific liver enzyme commonly used to detect hepatobiliary disease and possible alcohol abuse. Isolated elevations of this enzyme may be due to biochemical anomalies rather than the presence of pathology.
متن کاملDonor substrate specificity and thiol reduction of glutathione disulfide peroxidase.
By isolation of a mixed disulfide product of glutathione and cysteine, glutathione peroxidase was shown to be highly specific for only one donor substrate. Using the coupled assay of NADPH and yeast glutatione reductase, which is highly specific for flutathione disulfide, it was shown that the apparent inhibition of glutathione peroxidase by mercaptoethanol can be described kinetically and that...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemico-Biological Interactions
سال: 2013
ISSN: 0009-2797
DOI: 10.1016/j.cbi.2013.02.007